Penn Vet: New test can detect gene-doping therapies in equines

Posted: March 29, 2021

Researchers at the University of Pennsylvania School of Veterinary Medicine have successfully developed a new test to systemically detect the local administration of illicit, gene-doping therapies in equine athletes, the university reported in a recent press release.

Penn Vet said the findings from the novel study—supported in part by the Pennsylvania Horse Breeders Association and Pennsylvania Horse Racing Commission—represent “a significant breakthrough in the collective fight to advance the welfare and integrity of sport for both horses and humans.”

The team of researchers was led by Dr. Mary Robinson, Director of the Equine Pharmacology Laboratory at Penn Vet’s New Bolton Center. They created and validated a quantitative real-time polymerase chain reaction test, commonly known as a PCR test, that is able to detect the presence of a gene-doping agent in plasma and synovial fluid after its intra-articular administration in horses. Penn Vet said gene-doping agents trigger cells to produce performance enhancing proteins, which often are more elusive due to their virtually indistinguishable characteristics from naturally occurring proteins within the body.

“For the first time, we have demonstrated that a PCR test performed on a blood sample can detect the local administration of a gene therapy into the joint of a horse,” Robinson said. “While this test is currently limited in that it can only detect a specific gene therapy, it provides proof of concept that a gene therapy administered into the joint can be detected in a blood sample in a manner that is quick, convenient, and consistent with our long-term goal of deploying pre-race testing someday in the future.”

According to the release, Penn Vet researchers were able to detect the presence of the product in equine joint fluid after intra-articular administration of gene therapy, and they were also able to detect it in blood for up to 28 days. Penn Vet called that a “significantly robust window of time that could be useful for pre-race as well as out-of-competition testing.”

“The ability to detect the presence of these gene-doping agents in blood after local administration to joints just magnifies the implications of this game-changing development,” said Dr. Joanne Haughan, one of the lead investigators on the study. “The science is closing in on those who seek to use these advancements for wrongful means; the more we learn with each study, the harder it will be for individuals who seek to cheat the system using gene-doping strategies.”

The ongoing body of research in gene doping is being performed concurrently with Penn Vet’s larger multi-tiered, multi-year project to expand upon the New Bolton Center’s equine BioBank. Established in 2017 using internal funds from the Raymond Firestone Trust Research Grant and expanded in 2018 with support from the PHBA, the growing database collects and analyzes multiple types of samples, looking for a myriad of potential biomarkers in equine athletes. With the goal of creating biological passports, researchers believe the biomarkers could also be key in detecting gene doping as well as predicting injuries before they happen.

“As breeders, protecting the health, safety, and well-being of our horses is a deeply profound and personal priority for our membership,” said Brian Sanfratello, Executive Secretary of the PHBA. “These scientific discoveries get us one step closer to our dream of someday keeping equine sport completely clean. We are proud to support Dr. Robinson and her team of experts as they continue to incrementally drive us closer and closer to making that dream a reality.”

With the completion of a third study on the horizon, Penn Vet’s researchers seek to further expand and refine their testing methodology in order to create screening tests that would successfully identify multiple gene doping agents for even longer periods of time.

“We still have a lot of work to do to better understand the nature of bio-markers and how to fully harness their capabilities, but the science for detecting gene doping is getting there and much more quickly than any of us could have anticipated when we started this research,” Robinson said. “Ideas that once may have seemed unattainable–like a hand-held, stall-side testing device–are now coming into sight as real and tangible possibilities. We just need continued support to help get us there.”

(Photo courtesy of Penn Vet)